Results🔗

• a) 420 Up regulated and 165 Down regulated entities are obtained
• b) Gene Ontology has shown significantly enriched GO terms, and pathway analysis has shown significant gene expression differences in the following 22 pathways: p38 signalling mediated by MAPKAP kinases, P38 MAPK signalling pathway, validate targets of C-MYC transcriptional activation, VEGFR1 specific signals, TNF alpha/ NF-kB, TGFBR, Hypoxic and oxygen homeostasis, regulation of HIF-1-alpha, Endogenous TLR signalling, Direct Interactions, Cellular roles of Anthrax toxin, C-MYC pathway, BMP

receptor signalling, Androgen Receptor Androgen-mediated signalling, ATF-2 transcription factor network, signalling mediated by p38 alpha and p38-beta, Regulation of p38-alpha and p38-beta, Regulation of Androgen receptor activity, N0 cadherin signalling events, IL27 mediated signalling events and IL12 mediated signalling events ```

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• c) GSEA analysis for the dataset wherein Kaposi Liver cancer Poor Survival was found to be significant.
• d) Further analysis was done for cancer, autoimmune & neurotransmission related diseases and results are as below. MeSH pathway analysis was done for the following terms:
• a) Autoimmunity
• b) Neurotransmitter: Neurotransmitter Uptake, Inhibitors, Receptors and Synaptic Transmission together.
• c) Cancer: Brain, Liver and Neoplastic Stem Cells separately.

We limited the network building to these terms because including all of the cancer MeSH terms leads to large network.

Following are the observations made: Significant genes from Pathways which are two fold up or down regulated:

• with Autoimmunity: 18 with Neurotransmitter: 17 with Brain cancer: 40 with Liver cancer: 59 with Neoplastic Stem cells: 18 Interestingly 8 genes were common in all the cancer lists. 3(EIF4E, BMI1, TMEF2) of these were significant in C-MYC pathway as well. 5 out of 59 significant genes from liver cancer network are also found in IL 12 mediated signalling pathways, 7 in BMP receptor signalling, 3 in IL27, 5 in TGFBR, 4 in Androgen receptor and 8 in c-myc pathway.